Loss of heterozygosity (LOH) at polymorphic loci on the short arm of chromosome 3 which has multiple recurrence genes was investigated in histopathologically proven 35 sporadinc renal cell carcinoma (RCC) at Dong-A University. The allelic
frequencies
anf the rate of LOH applying a polymerase chain reaction (PCR) at five loci, D3S2 (MSPI), THRB ( EcoRI), THRB (BamHI), THRB (MSPI) an Dd3f15s2 (hINDⅢ) were investigated. The allelic frequencies were quite different between normal subjects and
patients
with RCC in Koreans. The rate of LOH was shown lower at all loci than those of Caucasians. The clear distinction in the incidence of LOH was shown between clear cell and granular cell type. LOH was not observed at THRB locus in clear cell tpe
whereasd
in granular cell type. LOH was not observed at THRB locus in clear cell type wheras in granual cell type, LOH could dnot be observedd at D3F15S2 locus. The following conclusion was derived. The author found that one allelic gfene was changed in
patients
swith RCC because the allelic frequencies were different between normal subjects and patients wth RCC. Also, the genetic characteristics in Koreans were different from Caucasians, and the detection of genetic loci specific for Koreans is
important
in
oncogenetic studies. Lastly, Allelic analysis wa scompared with the histopathological finding of genetic alterationsl so to correlate the relationship between oncogenesis and genetic alterations. Therefore, this ssutdy suggested that different
genes are
involvued in oncogenesis and genetic alterations. Therefore, this study suggested that different genes are involved in oncogenesis according to histopathological types.
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